Friction patterns guide actin network contraction.

A. Colin*, M. Orhant-Prioux*, C. Guérin*, M. Savinov*, I. Scarfone, A. Roux, E. M. De La Cruz, A. Mogilner, M. Théry*, L. Blanchoin*

Proceedings of National Academy of Science USA, 20(39):e2300416120, 2023.


Compressive forces stabilise microtubules in living cells.

Li Y, Kučera O, Cuvelier D, Rutkowski DM, Deygas M, Rai D, Pavlovič T, Vicente FN, Piel M, Giannone G, Vavylonis D, Akhmanova A, Blanchoin L*, Théry M*.

Nature Materials, 22(7):913-924, 2023.


Recycling of the actin monomer pool limits the lifetime of network turnover.

Colin A, Kotila T, Guérin C, Orhant-Prioux M, Vianay B, Mogilner A, Lappalainen P, Théry M*, Blanchoin L*.

EMBO Journal :e112717, 2023.


Microtubules self-repair in living cells.

Gazzola M, Schaeffer A, Butler-Hallissey C, Friedl K, Vianay B, Gaillard J, Leterrier C, Blanchoin L*, Théry M*.

Current Biology. 33(1):122-133.e4, 2023.



Actin architecture steers microtubules in active cytoskeletal composite.

Kučera O*, Gaillard J, Guérin C, Théry M*, Blanchoin L*.

Nanoletters, 22(21):8584-8591, 2022.


Actin network architecture can ensure robust centering or sensitive decentering of the centrosome.

Yamamoto S, Gaillard J, Vianay B, Guerin C, Orhant-Prioux M, Blanchoin L*, Théry M*.
EMBO Journal, 41(20):e111631, 2022.


Actin-microtubule dynamic composite forms responsive active matter with memory.

Kučera O, Gaillard J, Guérin C, Théry M*, Blanchoin L*.

Proc Natl Acad Sci U S A. ;119(31):e2209522119, 2022.


Flagella-like beating of actin bundles driven by self-organized myosin waves.

Pochitaloff M, Miranda M, Richard M, Chaiyasitdhi A, Takagi Y, Cao W, De La Cruz E, Sellers J, Joanny JF, Jülicher F, Blanchoin L, Martin P.

Nature Physics, 18(10):1240-1247, 2022


Reconstituting the Interaction Between Purified Nuclei and Microtubule Network.

Agsu G, Gaillard J, Cadot B, Blanchoin L, Fabre E*, Théry M*.

Methods Mol Biol. 2430:385-399, 2022


Visualization and Quantification of Microtubule Self-Repair.

Gaillard J, Blanchoin L, Théry M, Schaedel L.

Methods Mol Biol. 2430:279-289, 2022


Physical properties of the cytoplasm modulate the rates of microtubule polymerization and depolymerization.

Molines AT, Lemière J, Gazzola M, Steinmark IE, Edrington CH, Hsu CT, Real-Calderon P, Suhling K, Goshima G, Holt LJ, Théry M, Brouhard GJ, Chang F.

Developmental Cell, 57(4):466-479, 2022.


Microtubules tune mechanosensitive cell responses.

Seetharaman S, Vianay B, Roca V, Farrugia AJ, De Pascalis C, Boëda B, Dingli F, Loew D, Vassilopoulos S, Bershadsky A, Théry M, Etienne-Manneville S.

Nature Materials, 21,366–377, 2022.



Hematopoietic progenitors polarize in contact with bone marrow stromal cells by engaging CXCR4 receptors.

Bessy T, Candelas A,  Souquet B,  Saadallah K,  Schaeffer A, Vianay B,  Cuvelier D, Gobaa S, … Bories JC,  Mooney N, Jaffredo T, Larghero J, Blanchoin L,  Faivre L, Brunet S*, Théry M*.

The Journal of Cell Biology, 220(11):e202005085, 2021.


Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.

Krüger LK, Gélin M, Ji L, Kikuti C, Houdusse A, Théry M, Blanchoin L, Tran PT.

Elife. 10:e67489, 2021.


Manufacturing a Bone Marrow-On-A-Chip Using Maskless Photolithography.

Souquet B, Opitz M, Vianay B, Brunet S, Théry M.

Methods Molecular Biology, 2308:263-278, 2021.


The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces.

Kollimada S, Senger F, Vignaud T, Théry M*, Blanchoin L*, Kurzawa L*.

Molecular Biology of the Cell. 19;32(18):1737-1748. 2021.


Acto-myosin network geometry defines centrosome position.

Jimenez AJ, Schaeffer A, De Pascalis C, Letort G, Vianay B, Bornens M, Piel M, Blanchoin L, Théry M.

Current Biology, 31(6):1206-1220.e5, 2021.


Self-repair protects microtubules from their destruction by molecular motors.

Triclin S, Inoue D, Gaillard J, Htet ZM, DeSantis ME, Portran D, Derivery E, Aumeier C, Schaedel L, John K, Leterrier C, Reck-Peterson SL, Blanchoin L*, Théry M*.

Nature Materials, 20(6):883-891, 2021.


­Stress fibers are embedded in a contractile cortical network.

Vignaud T, Copos C, Leterrier C, Toro-Nahuelpan M, Tseng Q, Mahamid J, Blanchoin L, Mogilner A*, Théry M*, Kurzawa L*.

Nature Materials, 20(3):410-42, 2021.


Fat1 deletion promotes hybrid EMT state, tumour stemness and metastasis.

Pastushenko I, Mauri F, Song Y, de Cock F, Meeusen B, Swedlund B, Impens F, Van Haver D, Opitz M, Thery M, …, Pérez-Morga D, Salmon I, Sotiriou C, Helmbacher F, Blanpain C.

Nature. 589(7842): 448-455, 2021.



Microtubule self-repair.

Théry M* & Blanchoin L*.

Current Opinion in Cell Biology. 68:144-154, 2020.


Microtubules control nuclear shape and gene expression during early stages of

hematopoietic differentiation

Biedzinski S, Agsu G, Vianay B, Delord, Blanchoin L, Larghero J, Faivre L, Théry M*, Brunet S*.

EMBO Journal, 39:e103957, 2020.


CLASP mediates microtubule repair by restricting lattice damage and regulating tubulin incorporation.

Aher A, Rai D, Schaedel L, Gaillard J, John K, Liu Q, Altelaar M, Blanchoin L, Thery M, Akhmanova A.

Current Biology 30, 2175–2183, 2020.


Force Production by a Bundle of Growing Actin Filaments Is Limited by Its Mechanical Properties

Martiel JL, Michelot A, Boujemaa-Paterski R, Blanchoin L, Berro J.

Biophysical Journal, 118(1):182-192.



Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies.

Toro-Nahuelpan M, Zagoriy I, Senger F, Blanchoin L, Thery M, Mahamid J.

Nature Methods. 17(1):50-54, 2020.


Insights into the evolution of regulated actin dynamics via characterization of primitive gelsolin/cofilin proteins from Asgard archaea.

Akil C, Tran LT, Orhant-Prioux M, Baskaran Y, Manser E, Blanchoin L, Robinson RC

PNAS, 117(33):19904-19913.


Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion.

Damiano-Guercio J, Kurzawa L, Mueller J, Dimchev G, Schaks M, Nemethova M, Pokrant T, Brühmann S, Linkner J, Blanchoin L, Sixt M, Rottner K, Faix J

Elife, 9:e55351.



Spatial integration of mechanical forces by alpha-actinin establishes actin network symmetry

Journal of Cell Science, 14;132(22).

Senger F, Pitaval A, Ennomani H, Kurzawa L, Blanchoin L, Théry M.


Lattice defects induce microtubule self-renewal

Nature Physics, 15:830-838.

Schaedel L, Triclin S, Chrétien D, Abrieu A, Aumeier C, Gaillard J, Blanchoin L, Théry M, John K.


Active cargo positioning in antiparallel transport networks


Richard M, Blanch-Mercader C, Ennomani H, Cao W, De La Cruz EM, Joanny JF, Jülicher F, Blanchoin L, Martin P.


Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis

EMBO Journal, 3;38(11).

Farina F, Ramkumar N, Brown L, Samandar Eweis D, Anstatt J, Waring T, Bithell J, Scita G, Théry M, Blanchoin L, Zech T, Baum B.


Actin filaments regulate microtubule growth at the centrosome

EMBO Journal, 3;38(11).

 Inoue D, Obino D, Pineau J, Farina F, Gaillard J, Guerin C, Blanchoin L, Lennon-Duménil AM, Théry M.


Quantitative regulation of the dynamic steady state of actin networks

Elife. 14;8, e42413

Manhart A, Icheva A, Guerin C, Klar T, Boujemaa-Paterski R, Thery M, Blanchoin L, Mogilner A.



Dynamic stability of the actin ecosystem

Journal of Cell Science,132(4), pii: jcs219832.

Plastino J, Blanchoin L.


A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines

Nature Communications, 9(1):3775.

Peris L, … , Blanchoin L, Delphin C, Andrieux A.


Cellular stretch reveals superelastic powers

Nature. 563(7730):192-194. News and Views.

Théry M, Asnacios A.


Geometrical confinement controls the asymmetric patterning of Brachyury in cultures of pluripotent cells

Development, 145(18).

Blin G, Wisniewski D, Picart C, Thery M, Puceat M, Lowell S.


Actin-Network Architecture Regulates Microtubule Dynamics

Current Biology , 28(16):2647-2656.e4

Colin A, Singaravelu P, Théry M, Blanchoin L, Gueroui Z.


Variation in traction forces during cell cycle progression

Biology of the Cell, 110(4):91-96.

Vianay B, Senger F, Alamos S, Anjur-Dietrich M, Bearce E, Cheeseman B, Lee L, Théry M.



Stem Cell-Like Properties of CK2b-down Regulated Mammary Cells

Cancers, 9(9) pii: E114,

Duchemin-Pelletier E, Baulard M, Spreux E, Prioux M, Burute M, Mograbi B, Guyon L, Théry M, Cochet C, Filhol O.


Actin Filament Strain Promotes Severing and Cofilin Dissociation

Biophysical Journal. 112(12):2624-2633.

Schramm AC, Hocky GM, Voth GA, Blanchoin L, Martiel JL, De La Cruz EM.


Microtubule stabilization drives 3D centrosome migration to initiate primary ciliogenesis

The Journal of Cell Biology, 216(11):3713-3728

Pitaval A., Senger S, Letort G, Gidrol X, Guyon L, Sillibourne J, Théry M.


Network heterogeneity regulates steering in actin-based motility

Nature Communication, 8(1):655.

Boujemaa-Paterski R, Suarez C, Klar T, Zhu J, Guérin C, Mogilner A, Théry M, Blanchoin L


Adaptive Actin Networks

Developmental Cell, 42(6):565-566.

Plastino J, Blanchoin L.


Adaptive Response of Actin Bundles under Mechanical Stress

Biophys Journal, 113(5):1072-1079.

Rückerl F, Lenz M, Betz T, Manzi J, Martiel JL, Safouane M, Paterski-Boujemaa R, Blanchoin L, Sykes C.


Dissipation of contractile forces: the missing piece in cell mechanics

Molecular Biology of the Cell, 28(14):1825-1832, 2017.

Kurzawa L, Vianay B, Senger F, Vignaud T, Blanchoin L, Théry M.


The size-speed-force relationship governs migratory cell response to tumorigenic factors

Molecular Biology of the Cell, 28(12):1612-1621.

Leal-Egaña A, Letort G, Martiel JL, Christ A, Vignaud T, Roelants C, Filhol O, Théry M.


Microtubules acquire resistance from mechanical breakage through intralumenal acetylation

Science, 356(6335):328-332, 201

Xu Z, Schaedel L, Portran D, Aguilar A, Gaillard J, Marinkovich MP, Théry M, Nachury MV.


Tubulin acetylation protects long-lived microtubules against mechanical ageing

Nature Cell Biology, 19(4):391-398

Portran D, Schaedel L, Xu Z, Théry M and Nachury M.


Polarity reversal by centrosome repositioning primes cell scattering during epithelial-to-mesenchymal transition

Developmental Cell, 40 (2): 168-84, 201

 Burute M, Prioux M, Blin G, Truchet S, Letort G, Tseng Q, Bessy T, Lowell S, Young J, Filhol O, Théry M.



Self-repair promotes microtubule rescue

Nature Cell Biology, 18(10):1054-64

Aumeier C, Schaedel L, Gaillard J, John K, Blanchoin L and Théry M.


Centrosome centering and decentering by microtubule network rearrangement

Molecular Biology of the Cell. 27(18):2833-43

Letort G, Nedelec F, Blanchoin L, Théry M.


Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation

 Nature Cell Biology, 18(9):941-5

Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Théry M, Dunn AR, Fässler R.


Architecture and connectivity govern actin network contractility

Current Biology, 26(5):616-26.

Ennomani H, Letort G, Guérin C, Martiel JL, Cao W, Nédélec F, De La Cruz EM, Théry M, Blanchoin L.


Actin nucleation at the centrosome controls lymphocyte polarity

Nature Communications, 7:10969.

Obino D, Farina F, Malbec O, Sáez PJ, Maurin M, Gaillard J, Dingli F, Loew D, Gautreau A, Yuseff MI, Blanchoin L, Théry M, Lennon-Duménil AM.



The centrosome is an actin-organizing center

Nature Cell Biology, 18(1):65-75.

Farina F, Gaillard J, Guerin C, Couté Y, Sillibourne J, Blanchoin L, Théry M.


Microtubules self-repair in response to mechanical stress

Nature Materials, 14, 1156?1163

Schaedel L, John K, Gaillard J, Nachury MV, Blanchoin L and Théry M.


Geometrical and Mechanical Properties Control Actin Filament Organization

PLoS Comput Biol. 11(5):e1004245.

Letort G. , Politi A.Z., Ennomani H., Théry M., Nedelec F., Blanchoin L.


Architecture Dependence of Actin Filament Network Disassembly

Current Biology, 25 (11), p 1437?1447

Gressin L., Guillotin A., Guérin C., Blanchoin L. and Michelot A.


Design of a 2D no-flow chamber to monitor hematopoietic stem cells

Lab on a Chip, 15(1):77-85

Cambier T, Honegger T, Vanneaux V, Berthier J, Peyrade D, Blanchoin L, Larghero J, Théry M.



Working in the real and the imaginary

Molecular Biology of the Cell. 25(21):3248-50.

Théry M.


Cytokinesis failure triggers hippo tumor suppressor pathway activation

Cell. 158(4):833-48, 2014.

Ganem NJ, Cornils H, Chiu SY, O\’Rourke KP, Arnaud J, Yimlamai D, Théry M, Camargo FD, Pellman D.


Cell adhesion geometry regulates non-random DNA segregation and asymmetric cell fates in mouse skeletal muscle stem cells

Cell Reports, 7(4):961-70, 2014.

Yennek S, Burute M, Théry M and Tajbakhsh S.


Oncogene-like induction of cellular invasion from centrosome amplification

Nature, 510:167-71, 2014.

Godinho SA, Picone R, Burute M, Dagher R, Su Y, Leung CT, Polyak K, Brugge JS, Théry M and Pellman D.


Directed actin assembly and motility

Methods in Enzymology, 540:283-300, 2014.

Boujemaa-Paterski R, Galland R, Suarez C, Guérin C, Théry M* and Blanchoin L*.


Geometrical Control of Actin Assembly and Contractility

Methods in Cell Biology, 120:19-38, 2014.

Reymann AC, Guérin C, Théry M, Blanchoin L and Boujemaa-Paterski R.


Polyacrylamide hydrogel micropatterning

Methods in Cell Biology, 120:93-116, 2014.

Vignaud T, Ennomani H, Théry M.


Actin dynamics, architecture, and mechanics in cell motility

Physiol Rev. 2014 Jan;94(1):235-63

Blanchoin L, Boujemaa-Paterski R, Sykes C, Plastino J.



Probing ciliogenesis using micropatterned substrates

Methods in Enzymology, 525 : 109-130, 2013.

Pitaval A., Christ A., Curtet A., Tseng Q., Théry M.


Beta1- and AlphaV-class integrins cooperate to regulate myosin-II during

Nature Cell Biology, 15(6):625-36, 2013.

Schiller HB, Hermann M, Polleux J, Vignaud T, Zanivan S, Friedel CC, Sun Z, Raducanu A, Gottschalk K, Théry M, Mann M and Fässler R


Microtubule sliding activity of a kinesin-8 promotes spindle assembly and spindle length control

Nature Cell Biology, 15(8):948-57, 2013.

Su X, Arellano-Santoyo H, Portran D, Gaillard J, Vantard M, Théry M and Pellman D.


Human Bone Marrow Mesenchymal Stem Cells Regulate Biased DNA Segregation in Response to Cell Adhesion Asymmetry

Cell Reports, 14;5(3):601-10

Freida D, Lecourt S, Cras A, Vanneaux V, Letort G, Gidrol X, Guyon L, Larghero J and Théry M.


Inhibitory signalling to the Arp2/3 complex steers cell migration

Nature. 2013;503:281-4

Dang I, Gorelik R, Sousa-Blin C, Derivery E, Guérin C, …, Blanchoin L, Gautreau A


Quantification of MAP and molecular motor activities on geometrically controlled microtubule networks

Cytoskeleton. 70(1) : 12-23, 2013

Portran D, Gaillard J, Vantard M, Thery M.


Fabrication of three-dimensional electrical connections by means of directed actin self-organization

Nature Materials, 12 : 416?421, 2013.

Galland R, Leduc P, Guérin C, Peyrade D, Blanchoin L, Thery M



How actin network dynamics control the onset of actin-based motility

PNAS, 109(36):14440-5

Kawska A, Carvalho K, Manzi J, Boujemaa-Paterski R, Blanchoin L, Martiel JL, Sykes C.


Spatial organization of the extra-cellular matrix regulates cell-cell junction positioning

Proceedings of National Academy of Science U S A. 109 (5) 1506-1511, 2012.

Tseng Q, Duchemin-Pelletier E, Deshiere A, Balland M, Guillou H, Filhol O, Thery M.


Reprogramming cell shape with live nano-patterning

Journal of Cell Science, 125:2134-2140, 2012.

Vignaud T, Galland R, Tseng Q, Blanchoin L, Colombelli J, Thery M.


The first world cell race

Current Biology. 2012 Sep 11;22(17):R673-5.

Maiuri P, Terriac E, Paul-Gilloteaux P, Vignaud T, McNally K, Onuffer J, Thorn K, Nguyen PA, Georgoulia N, Soong D, Jayo A, Beil N.


Spatial segregation between cell-cell and cell-matrix adhesions

Current Opinion in Cell Biology. 24(5):628-36, 2012.

Burute M, Théry M.


Actin network architecture determines myosin motor activity

Science, 336(6086):1310-4, 2012.

Reymann AC, Boujemaa-Paterski R, Martiel JL, Guérin C, Cao W, Chin HF, De La Cruz E, Thery M , Blanchoin L.


Directed cytoskeleton self-organization

Trends Cell Biology, 22(12) : 671-682, 2012.

Vignaud T, Blanchoin L, Thery M


Before 2012

A new micropatterning method of soft substrates reveals that different tumorigenic signals can promote or reduce cell contraction levels

Lab On Chip, 11:2231-40, 2011.

Tseng Q, Wang I, Duchemin-Pelletier E, Azioune A, Carpi N, Gao J, Filhol O, Piel M, Thery M


Turnover of branched actin filament networks by stochastic fragmentation with ADF/cofilin

Molecular Biology of the Cell. 22(14):2541-50, 2011

Reymann AC, Suarez C, Guérin C, Martiel JL, Staiger CJ, Blanchoin L*, Boujemaa-Paterski R*.


Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes

Genes and Development, 22(16):2189-203, 2008

Kwon M, Godinho SA, Chandhok NS, Ganem NJ, Azioune A, Thery M, Pellman D.


Actin filament dynamics are dominated by rapid growth and severing activity in the Arabidopsis cortical array

The Journal of Cell Biology. 184(2):269-80, 2009

Staiger CJ, Sheahan MB, Khurana P, Wang X, McCurdy DW, Blanchoin L.


Cell shape and contractility regulate ciliogenesis in cell cycle arrested cells

Journal of Cell Biology, 191(2): 303-12, 2010.

Pitaval A, Tseng Q, Bornens M, Thery M.


Nucleation geometry governs ordered actin networks structures

Nature Materials, 9(10):827-32, 2010.

Reymann AC, Martiel JL, Cambier T, Blanchoin L, Boujemaa-Paterski R and Thery M.


Cell micropatterning as a tool to decipher cell morphogenesis and functions

Journal of Cell Science, 123: 4201-4213, 2010. Review.

Thery M.


A ~primer~ -based mechanism underlies branched actin filament network formation and motility

Current Biology. 9;20(5):423-8, 2010.

Achard V, Martiel JL, Michelot A, Guérin C, Reymann AC, Blanchoin L*, Boujemaa-Paterski R*.


Experimental and theoretical study of mitotic spindle orientation

Nature, 447(7143): 493-6, 2007.

Thery M, Jimenez-Dalmaroni A, Racine V, Bornens M and Julicher F.


Actin-filament stochastic dynamics mediated by ADF/cofilin

Current Biology, 17(10):825-33, 2007.

Michelot A, Berro J, Guérin C, Boujemaa-Paterski R, Staiger CJ, Martiel JL, Blanchoin L.


Cell distribution of stress fibres in response to the geometry of the adhesive environment

Cell Motility and the Cytoskeleton, 63(6):341-55, 2006.

Thery M, Pepin A, Dressaire E, Chen Y, Bornens M.


Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity

Proceedings of National Academy of Science U S A. 103(52):19771-6, 2006.

Thery M, Racine V, Piel M, Pepin A, Dimitrov A, Chen Y, Sibarita JB, Bornens M.


A novel mechanism for the formation of actin-filament bundles by a nonprocessive formin

Current Biology. 16(19):1924-30, 2006

Michelot A, Derivery E, Paterski-Boujemaa R, Guérin C, Huang S, Parcy F, Staiger CJ, Blanchoin L.


The extracellular matrix guides the orientation of the cell division axis

Nature Cell Biology, 7(10):947-53, 2005.

Thery M, Racine V, Pepin A, Piel M, Chen Y, Sibarita JB, Bornens M.


Plant formin AtFH5 is an evolutionarily conserved actin nucleator involved in cytokinesis

Nature Cell Biology. 7(4):374-80, 2005.

Ingouff M, Fitz Gerald JN, Guérin C, Robert H, Sørensen MB, Van Damme D, Geelen D, Blanchoin L, Berger F.


Interactions of ADF/cofilin, Arp2/3 complex, capping protein and profilin in remodeling of branched actin filament networks

Current Biology, 10(20):1273-82, 2000.

Blanchoin L, Pollard TD, Mullins RD.


Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins

Nature, 404(6781):1007-11, 2000.

Blanchoin L, Amann KJ, Higgs HN, Marchand JB, Kaiser DA, Pollard TD.


Molecular mechanisms controlling actin filament dynamics in nonmuscle cells

Annu Rev Biophys Biomol Struct, 29:545-76, 2000

Pollard TD, Blanchoin L, Mullins RD.