2023
Friction patterns guide actin network contraction.
A. Colin*, M. Orhant-Prioux*, C. Guérin*, M. Savinov*, I. Scarfone, A. Roux, E. M. De La Cruz, A. Mogilner, M. Théry*, L. Blanchoin*
Proceedings of National Academy of Science USA, 20(39):e2300416120, 2023.
Compressive forces stabilise microtubules in living cells.
Li Y, Kučera O, Cuvelier D, Rutkowski DM, Deygas M, Rai D, Pavlovič T, Vicente FN, Piel M, Giannone G, Vavylonis D, Akhmanova A, Blanchoin L*, Théry M*.
Nature Materials, 22(7):913-924, 2023.
Recycling of the actin monomer pool limits the lifetime of network turnover.
Colin A, Kotila T, Guérin C, Orhant-Prioux M, Vianay B, Mogilner A, Lappalainen P, Théry M*, Blanchoin L*.
EMBO Journal :e112717, 2023.
Microtubules self-repair in living cells.
Gazzola M, Schaeffer A, Butler-Hallissey C, Friedl K, Vianay B, Gaillard J, Leterrier C, Blanchoin L*, Théry M*.
Current Biology. 33(1):122-133.e4, 2023.
2022
Actin architecture steers microtubules in active cytoskeletal composite.
Kučera O*, Gaillard J, Guérin C, Théry M*, Blanchoin L*.
Nanoletters, 22(21):8584-8591, 2022.
Actin network architecture can ensure robust centering or sensitive decentering of the centrosome.
Yamamoto S, Gaillard J, Vianay B, Guerin C, Orhant-Prioux M, Blanchoin L*, Théry M*.
EMBO Journal, 41(20):e111631, 2022.
Actin-microtubule dynamic composite forms responsive active matter with memory.
Kučera O, Gaillard J, Guérin C, Théry M*, Blanchoin L*.
Proc Natl Acad Sci U S A. ;119(31):e2209522119, 2022.
Flagella-like beating of actin bundles driven by self-organized myosin waves.
Pochitaloff M, Miranda M, Richard M, Chaiyasitdhi A, Takagi Y, Cao W, De La Cruz E, Sellers J, Joanny JF, Jülicher F, Blanchoin L, Martin P.
Nature Physics, 18(10):1240-1247, 2022
Reconstituting the Interaction Between Purified Nuclei and Microtubule Network.
Agsu G, Gaillard J, Cadot B, Blanchoin L, Fabre E*, Théry M*.
Methods Mol Biol. 2430:385-399, 2022
Visualization and Quantification of Microtubule Self-Repair.
Gaillard J, Blanchoin L, Théry M, Schaedel L.
Methods Mol Biol. 2430:279-289, 2022
Physical properties of the cytoplasm modulate the rates of microtubule polymerization and depolymerization.
Molines AT, Lemière J, Gazzola M, Steinmark IE, Edrington CH, Hsu CT, Real-Calderon P, Suhling K, Goshima G, Holt LJ, Théry M, Brouhard GJ, Chang F.
Developmental Cell, 57(4):466-479, 2022.
Microtubules tune mechanosensitive cell responses.
Seetharaman S, Vianay B, Roca V, Farrugia AJ, De Pascalis C, Boëda B, Dingli F, Loew D, Vassilopoulos S, Bershadsky A, Théry M, Etienne-Manneville S.
Nature Materials, 21,366–377, 2022.
2021
Hematopoietic progenitors polarize in contact with bone marrow stromal cells by engaging CXCR4 receptors.
Bessy T, Candelas A, Souquet B, Saadallah K, Schaeffer A, Vianay B, Cuvelier D, Gobaa S, … Bories JC, Mooney N, Jaffredo T, Larghero J, Blanchoin L, Faivre L, Brunet S*, Théry M*.
The Journal of Cell Biology, 220(11):e202005085, 2021.
Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.
Krüger LK, Gélin M, Ji L, Kikuti C, Houdusse A, Théry M, Blanchoin L, Tran PT.
Elife. 10:e67489, 2021.
Manufacturing a Bone Marrow-On-A-Chip Using Maskless Photolithography.
Souquet B, Opitz M, Vianay B, Brunet S, Théry M.
Methods Molecular Biology, 2308:263-278, 2021.
The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces.
Kollimada S, Senger F, Vignaud T, Théry M*, Blanchoin L*, Kurzawa L*.
Molecular Biology of the Cell. 19;32(18):1737-1748. 2021.
Acto-myosin network geometry defines centrosome position.
Jimenez AJ, Schaeffer A, De Pascalis C, Letort G, Vianay B, Bornens M, Piel M, Blanchoin L, Théry M.
Current Biology, 31(6):1206-1220.e5, 2021.
Self-repair protects microtubules from their destruction by molecular motors.
Triclin S, Inoue D, Gaillard J, Htet ZM, DeSantis ME, Portran D, Derivery E, Aumeier C, Schaedel L, John K, Leterrier C, Reck-Peterson SL, Blanchoin L*, Théry M*.
Nature Materials, 20(6):883-891, 2021.
Stress fibers are embedded in a contractile cortical network.
Vignaud T, Copos C, Leterrier C, Toro-Nahuelpan M, Tseng Q, Mahamid J, Blanchoin L, Mogilner A*, Théry M*, Kurzawa L*.
Nature Materials, 20(3):410-42, 2021.
Fat1 deletion promotes hybrid EMT state, tumour stemness and metastasis.
Pastushenko I, Mauri F, Song Y, de Cock F, Meeusen B, Swedlund B, Impens F, Van Haver D, Opitz M, Thery M, …, Pérez-Morga D, Salmon I, Sotiriou C, Helmbacher F, Blanpain C.
Nature. 589(7842): 448-455, 2021.
2020
Microtubule self-repair.
Théry M* & Blanchoin L*.
Current Opinion in Cell Biology. 68:144-154, 2020.
Microtubules control nuclear shape and gene expression during early stages of
hematopoietic differentiation
Biedzinski S, Agsu G, Vianay B, Delord, Blanchoin L, Larghero J, Faivre L, Théry M*, Brunet S*.
EMBO Journal, 39:e103957, 2020.
CLASP mediates microtubule repair by restricting lattice damage and regulating tubulin incorporation.
Aher A, Rai D, Schaedel L, Gaillard J, John K, Liu Q, Altelaar M, Blanchoin L, Thery M, Akhmanova A.
Current Biology 30, 2175–2183, 2020.
Force Production by a Bundle of Growing Actin Filaments Is Limited by Its Mechanical Properties
Martiel JL, Michelot A, Boujemaa-Paterski R, Blanchoin L, Berro J.
Biophysical Journal, 118(1):182-192.
Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies.
Toro-Nahuelpan M, Zagoriy I, Senger F, Blanchoin L, Thery M, Mahamid J.
Nature Methods. 17(1):50-54, 2020.
Insights into the evolution of regulated actin dynamics via characterization of primitive gelsolin/cofilin proteins from Asgard archaea.
Akil C, Tran LT, Orhant-Prioux M, Baskaran Y, Manser E, Blanchoin L, Robinson RC
PNAS, 117(33):19904-19913.
Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion.
Damiano-Guercio J, Kurzawa L, Mueller J, Dimchev G, Schaks M, Nemethova M, Pokrant T, Brühmann S, Linkner J, Blanchoin L, Sixt M, Rottner K, Faix J
Elife, 9:e55351.
2019
Spatial integration of mechanical forces by alpha-actinin establishes actin network symmetry
Journal of Cell Science, 14;132(22).
Senger F, Pitaval A, Ennomani H, Kurzawa L, Blanchoin L, Théry M.
Lattice defects induce microtubule self-renewal
Nature Physics, 15:830-838.
Schaedel L, Triclin S, Chrétien D, Abrieu A, Aumeier C, Gaillard J, Blanchoin L, Théry M, John K.
Active cargo positioning in antiparallel transport networks
PNAS,116(30):14835-14842.
Richard M, Blanch-Mercader C, Ennomani H, Cao W, De La Cruz EM, Joanny JF, Jülicher F, Blanchoin L, Martin P.
Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis
EMBO Journal, 3;38(11).
Farina F, Ramkumar N, Brown L, Samandar Eweis D, Anstatt J, Waring T, Bithell J, Scita G, Théry M, Blanchoin L, Zech T, Baum B.
Actin filaments regulate microtubule growth at the centrosome
EMBO Journal, 3;38(11).
Inoue D, Obino D, Pineau J, Farina F, Gaillard J, Guerin C, Blanchoin L, Lennon-Duménil AM, Théry M.
Quantitative regulation of the dynamic steady state of actin networks
Elife. 14;8, e42413
Manhart A, Icheva A, Guerin C, Klar T, Boujemaa-Paterski R, Thery M, Blanchoin L, Mogilner A.
2018
Dynamic stability of the actin ecosystem
Journal of Cell Science,132(4), pii: jcs219832.
Plastino J, Blanchoin L.
A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
Nature Communications, 9(1):3775.
Peris L, … , Blanchoin L, Delphin C, Andrieux A.
Cellular stretch reveals superelastic powers
Nature. 563(7730):192-194. News and Views.
Théry M, Asnacios A.
Geometrical confinement controls the asymmetric patterning of Brachyury in cultures of pluripotent cells
Development, 145(18).
Blin G, Wisniewski D, Picart C, Thery M, Puceat M, Lowell S.
Actin-Network Architecture Regulates Microtubule Dynamics
Current Biology , 28(16):2647-2656.e4
Colin A, Singaravelu P, Théry M, Blanchoin L, Gueroui Z.
Variation in traction forces during cell cycle progression
Biology of the Cell, 110(4):91-96.
Vianay B, Senger F, Alamos S, Anjur-Dietrich M, Bearce E, Cheeseman B, Lee L, Théry M.
2017
Stem Cell-Like Properties of CK2b-down Regulated Mammary Cells
Cancers, 9(9) pii: E114,
Duchemin-Pelletier E, Baulard M, Spreux E, Prioux M, Burute M, Mograbi B, Guyon L, Théry M, Cochet C, Filhol O.
Actin Filament Strain Promotes Severing and Cofilin Dissociation
Biophysical Journal. 112(12):2624-2633.
Schramm AC, Hocky GM, Voth GA, Blanchoin L, Martiel JL, De La Cruz EM.
Microtubule stabilization drives 3D centrosome migration to initiate primary ciliogenesis
The Journal of Cell Biology, 216(11):3713-3728
Pitaval A., Senger S, Letort G, Gidrol X, Guyon L, Sillibourne J, Théry M.
Network heterogeneity regulates steering in actin-based motility
Nature Communication, 8(1):655.
Boujemaa-Paterski R, Suarez C, Klar T, Zhu J, Guérin C, Mogilner A, Théry M, Blanchoin L
Adaptive Response of Actin Bundles under Mechanical Stress
Biophys Journal, 113(5):1072-1079.
Rückerl F, Lenz M, Betz T, Manzi J, Martiel JL, Safouane M, Paterski-Boujemaa R, Blanchoin L, Sykes C.
Dissipation of contractile forces: the missing piece in cell mechanics
Molecular Biology of the Cell, 28(14):1825-1832, 2017.
Kurzawa L, Vianay B, Senger F, Vignaud T, Blanchoin L, Théry M.
The size-speed-force relationship governs migratory cell response to tumorigenic factors
Molecular Biology of the Cell, 28(12):1612-1621.
Leal-Egaña A, Letort G, Martiel JL, Christ A, Vignaud T, Roelants C, Filhol O, Théry M.
Microtubules acquire resistance from mechanical breakage through intralumenal acetylation
Science, 356(6335):328-332, 201
Xu Z, Schaedel L, Portran D, Aguilar A, Gaillard J, Marinkovich MP, Théry M, Nachury MV.
Tubulin acetylation protects long-lived microtubules against mechanical ageing
Nature Cell Biology, 19(4):391-398
Portran D, Schaedel L, Xu Z, Théry M and Nachury M.
Polarity reversal by centrosome repositioning primes cell scattering during epithelial-to-mesenchymal transition
Developmental Cell, 40 (2): 168-84, 201
Burute M, Prioux M, Blin G, Truchet S, Letort G, Tseng Q, Bessy T, Lowell S, Young J, Filhol O, Théry M.
2016
Self-repair promotes microtubule rescue
Nature Cell Biology, 18(10):1054-64
Aumeier C, Schaedel L, Gaillard J, John K, Blanchoin L and Théry M.
Centrosome centering and decentering by microtubule network rearrangement
Molecular Biology of the Cell. 27(18):2833-43
Letort G, Nedelec F, Blanchoin L, Théry M.
Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation
Nature Cell Biology, 18(9):941-5
Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Théry M, Dunn AR, Fässler R.
Architecture and connectivity govern actin network contractility
Current Biology, 26(5):616-26.
Ennomani H, Letort G, Guérin C, Martiel JL, Cao W, Nédélec F, De La Cruz EM, Théry M, Blanchoin L.
Actin nucleation at the centrosome controls lymphocyte polarity
Nature Communications, 7:10969.
Obino D, Farina F, Malbec O, Sáez PJ, Maurin M, Gaillard J, Dingli F, Loew D, Gautreau A, Yuseff MI, Blanchoin L, Théry M, Lennon-Duménil AM.
2015
The centrosome is an actin-organizing center
Nature Cell Biology, 18(1):65-75.
Farina F, Gaillard J, Guerin C, Couté Y, Sillibourne J, Blanchoin L, Théry M.
Microtubules self-repair in response to mechanical stress
Nature Materials, 14, 1156?1163
Schaedel L, John K, Gaillard J, Nachury MV, Blanchoin L and Théry M.
Geometrical and Mechanical Properties Control Actin Filament Organization
PLoS Comput Biol. 11(5):e1004245.
Letort G. , Politi A.Z., Ennomani H., Théry M., Nedelec F., Blanchoin L.
Architecture Dependence of Actin Filament Network Disassembly
Current Biology, 25 (11), p 1437?1447
Gressin L., Guillotin A., Guérin C., Blanchoin L. and Michelot A.
Design of a 2D no-flow chamber to monitor hematopoietic stem cells
Lab on a Chip, 15(1):77-85
Cambier T, Honegger T, Vanneaux V, Berthier J, Peyrade D, Blanchoin L, Larghero J, Théry M.
2014
Working in the real and the imaginary
Molecular Biology of the Cell. 25(21):3248-50.
Théry M.
Cytokinesis failure triggers hippo tumor suppressor pathway activation
Cell. 158(4):833-48, 2014.
Ganem NJ, Cornils H, Chiu SY, O\’Rourke KP, Arnaud J, Yimlamai D, Théry M, Camargo FD, Pellman D.
Cell adhesion geometry regulates non-random DNA segregation and asymmetric cell fates in mouse skeletal muscle stem cells
Cell Reports, 7(4):961-70, 2014.
Yennek S, Burute M, Théry M and Tajbakhsh S.
Oncogene-like induction of cellular invasion from centrosome amplification
Nature, 510:167-71, 2014.
Godinho SA, Picone R, Burute M, Dagher R, Su Y, Leung CT, Polyak K, Brugge JS, Théry M and Pellman D.
Directed actin assembly and motility
Methods in Enzymology, 540:283-300, 2014.
Boujemaa-Paterski R, Galland R, Suarez C, Guérin C, Théry M* and Blanchoin L*.
Geometrical Control of Actin Assembly and Contractility
Methods in Cell Biology, 120:19-38, 2014.
Reymann AC, Guérin C, Théry M, Blanchoin L and Boujemaa-Paterski R.
Polyacrylamide hydrogel micropatterning
Methods in Cell Biology, 120:93-116, 2014.
Vignaud T, Ennomani H, Théry M.
Actin dynamics, architecture, and mechanics in cell motility
Physiol Rev. 2014 Jan;94(1):235-63
Blanchoin L, Boujemaa-Paterski R, Sykes C, Plastino J.
2013
Probing ciliogenesis using micropatterned substrates
Methods in Enzymology, 525 : 109-130, 2013.
Pitaval A., Christ A., Curtet A., Tseng Q., Théry M.
Beta1- and AlphaV-class integrins cooperate to regulate myosin-II during
Nature Cell Biology, 15(6):625-36, 2013.
Schiller HB, Hermann M, Polleux J, Vignaud T, Zanivan S, Friedel CC, Sun Z, Raducanu A, Gottschalk K, Théry M, Mann M and Fässler R
Microtubule sliding activity of a kinesin-8 promotes spindle assembly and spindle length control
Nature Cell Biology, 15(8):948-57, 2013.
Su X, Arellano-Santoyo H, Portran D, Gaillard J, Vantard M, Théry M and Pellman D.
Human Bone Marrow Mesenchymal Stem Cells Regulate Biased DNA Segregation in Response to Cell Adhesion Asymmetry
Cell Reports, 14;5(3):601-10
Freida D, Lecourt S, Cras A, Vanneaux V, Letort G, Gidrol X, Guyon L, Larghero J and Théry M.
Inhibitory signalling to the Arp2/3 complex steers cell migration
Nature. 2013;503:281-4
Dang I, Gorelik R, Sousa-Blin C, Derivery E, Guérin C, …, Blanchoin L, Gautreau A
Quantification of MAP and molecular motor activities on geometrically controlled microtubule networks
Cytoskeleton. 70(1) : 12-23, 2013
Portran D, Gaillard J, Vantard M, Thery M.
Fabrication of three-dimensional electrical connections by means of directed actin self-organization
Nature Materials, 12 : 416?421, 2013.
Galland R, Leduc P, Guérin C, Peyrade D, Blanchoin L, Thery M
2012
How actin network dynamics control the onset of actin-based motility
PNAS, 109(36):14440-5
Kawska A, Carvalho K, Manzi J, Boujemaa-Paterski R, Blanchoin L, Martiel JL, Sykes C.
Spatial organization of the extra-cellular matrix regulates cell-cell junction positioning
Proceedings of National Academy of Science U S A. 109 (5) 1506-1511, 2012.
Tseng Q, Duchemin-Pelletier E, Deshiere A, Balland M, Guillou H, Filhol O, Thery M.
Reprogramming cell shape with live nano-patterning
Journal of Cell Science, 125:2134-2140, 2012.
Vignaud T, Galland R, Tseng Q, Blanchoin L, Colombelli J, Thery M.
The first world cell race
Current Biology. 2012 Sep 11;22(17):R673-5.
Maiuri P, Terriac E, Paul-Gilloteaux P, Vignaud T, McNally K, Onuffer J, Thorn K, Nguyen PA, Georgoulia N, Soong D, Jayo A, Beil N.
Spatial segregation between cell-cell and cell-matrix adhesions
Current Opinion in Cell Biology. 24(5):628-36, 2012.
Burute M, Théry M.
Actin network architecture determines myosin motor activity
Science, 336(6086):1310-4, 2012.
Reymann AC, Boujemaa-Paterski R, Martiel JL, Guérin C, Cao W, Chin HF, De La Cruz E, Thery M , Blanchoin L.
Directed cytoskeleton self-organization
Trends Cell Biology, 22(12) : 671-682, 2012.
Vignaud T, Blanchoin L, Thery M
Before 2012
A new micropatterning method of soft substrates reveals that different tumorigenic signals can promote or reduce cell contraction levels
Lab On Chip, 11:2231-40, 2011.
Tseng Q, Wang I, Duchemin-Pelletier E, Azioune A, Carpi N, Gao J, Filhol O, Piel M, Thery M
Turnover of branched actin filament networks by stochastic fragmentation with ADF/cofilin
Molecular Biology of the Cell. 22(14):2541-50, 2011
Reymann AC, Suarez C, Guérin C, Martiel JL, Staiger CJ, Blanchoin L*, Boujemaa-Paterski R*.
Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes
Genes and Development, 22(16):2189-203, 2008
Kwon M, Godinho SA, Chandhok NS, Ganem NJ, Azioune A, Thery M, Pellman D.
Actin filament dynamics are dominated by rapid growth and severing activity in the Arabidopsis cortical array
The Journal of Cell Biology. 184(2):269-80, 2009
Staiger CJ, Sheahan MB, Khurana P, Wang X, McCurdy DW, Blanchoin L.
Cell shape and contractility regulate ciliogenesis in cell cycle arrested cells
Journal of Cell Biology, 191(2): 303-12, 2010.
Pitaval A, Tseng Q, Bornens M, Thery M.
Nucleation geometry governs ordered actin networks structures
Nature Materials, 9(10):827-32, 2010.
Reymann AC, Martiel JL, Cambier T, Blanchoin L, Boujemaa-Paterski R and Thery M.
Cell micropatterning as a tool to decipher cell morphogenesis and functions
Journal of Cell Science, 123: 4201-4213, 2010. Review.
Thery M.
A ~primer~ -based mechanism underlies branched actin filament network formation and motility
Current Biology. 9;20(5):423-8, 2010.
Achard V, Martiel JL, Michelot A, Guérin C, Reymann AC, Blanchoin L*, Boujemaa-Paterski R*.
Experimental and theoretical study of mitotic spindle orientation
Nature, 447(7143): 493-6, 2007.
Thery M, Jimenez-Dalmaroni A, Racine V, Bornens M and Julicher F.
Actin-filament stochastic dynamics mediated by ADF/cofilin
Current Biology, 17(10):825-33, 2007.
Michelot A, Berro J, Guérin C, Boujemaa-Paterski R, Staiger CJ, Martiel JL, Blanchoin L.
Cell distribution of stress fibres in response to the geometry of the adhesive environment
Cell Motility and the Cytoskeleton, 63(6):341-55, 2006.
Thery M, Pepin A, Dressaire E, Chen Y, Bornens M.
Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity
Proceedings of National Academy of Science U S A. 103(52):19771-6, 2006.
Thery M, Racine V, Piel M, Pepin A, Dimitrov A, Chen Y, Sibarita JB, Bornens M.
A novel mechanism for the formation of actin-filament bundles by a nonprocessive formin
Current Biology. 16(19):1924-30, 2006
Michelot A, Derivery E, Paterski-Boujemaa R, Guérin C, Huang S, Parcy F, Staiger CJ, Blanchoin L.
The extracellular matrix guides the orientation of the cell division axis
Nature Cell Biology, 7(10):947-53, 2005.
Thery M, Racine V, Pepin A, Piel M, Chen Y, Sibarita JB, Bornens M.
Plant formin AtFH5 is an evolutionarily conserved actin nucleator involved in cytokinesis
Nature Cell Biology. 7(4):374-80, 2005.
Ingouff M, Fitz Gerald JN, Guérin C, Robert H, Sørensen MB, Van Damme D, Geelen D, Blanchoin L, Berger F.
Interactions of ADF/cofilin, Arp2/3 complex, capping protein and profilin in remodeling of branched actin filament networks
Current Biology, 10(20):1273-82, 2000.
Blanchoin L, Pollard TD, Mullins RD.
Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins
Nature, 404(6781):1007-11, 2000.
Blanchoin L, Amann KJ, Higgs HN, Marchand JB, Kaiser DA, Pollard TD.
Molecular mechanisms controlling actin filament dynamics in nonmuscle cells
Annu Rev Biophys Biomol Struct, 29:545-76, 2000
Pollard TD, Blanchoin L, Mullins RD.