{"id":3708,"date":"2022-09-08T16:24:44","date_gmt":"2022-09-08T14:24:44","guid":{"rendered":"http:\/\/cytomorpholab.com\/?page_id=3708"},"modified":"2022-09-08T16:30:22","modified_gmt":"2022-09-08T14:30:22","slug":"anr-thema","status":"publish","type":"page","link":"http:\/\/cytomorpholab.com\/index.php\/anr-thema\/","title":{"rendered":"ANR THEMA"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"3708\" class=\"elementor elementor-3708\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-77f0e13 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"77f0e13\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-468ea0f\" data-id=\"468ea0f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2cc5ed8 elementor-widget elementor-widget-text-editor\" data-id=\"2cc5ed8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.18.0 - 20-12-2023 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-aee81dd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"aee81dd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-fb3d591\" data-id=\"fb3d591\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-31540ec elementor-widget elementor-widget-image\" data-id=\"31540ec\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.18.0 - 20-12-2023 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t\t\t<img src=\"http:\/\/cytomorpholab.com\/wp-content\/uploads\/elementor\/thumbs\/logos-ANR_2022-pugo6ki60xr141aqjlwkegg9k9u99kb9ji8383hnyq.jpg\" title=\"logos-ANR_2022\" alt=\"logos-ANR_2022\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-66 elementor-top-column elementor-element elementor-element-1bab9a7\" data-id=\"1bab9a7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4f883c1 elementor-widget elementor-widget-text-editor\" data-id=\"4f883c1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h4>Agence Nationale pour la Recherche<\/h4>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d1232ec elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d1232ec\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1b2d9d1\" data-id=\"1b2d9d1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f34db53 elementor-widget elementor-widget-text-editor\" data-id=\"f34db53\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h4>18-IBHU-0003 (2019-2026)<\/h4>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d0eefa8 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d0eefa8\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f34cd58\" data-id=\"f34cd58\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-41c627d elementor-widget elementor-widget-text-editor\" data-id=\"41c627d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h4>THEMA \u2013 Leukemia on Chip<\/h4>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-055b722 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"055b722\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-4ebf7b7\" data-id=\"4ebf7b7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-469bed8 elementor-widget elementor-widget-image\" data-id=\"469bed8\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" width=\"800\" height=\"450\" src=\"http:\/\/cytomorpholab.com\/wp-content\/uploads\/2022\/09\/ANR-Schema-3.jpg\" class=\"attachment-large size-large wp-image-3408\" alt=\"\" srcset=\"http:\/\/cytomorpholab.com\/wp-content\/uploads\/2022\/09\/ANR-Schema-3.jpg 900w, http:\/\/cytomorpholab.com\/wp-content\/uploads\/2022\/09\/ANR-Schema-3-300x169.jpg 300w, http:\/\/cytomorpholab.com\/wp-content\/uploads\/2022\/09\/ANR-Schema-3-768x432.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-48a39d5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"48a39d5\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-a118e73\" data-id=\"a118e73\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e5273ad elementor-widget elementor-widget-text-editor\" data-id=\"e5273ad\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>The global aim of this project is to decipher the cellular and molecular mechanisms regulating the interaction of leukemic blasts with bone-marrow stromal cells. We have recently found that hematopoietic stem cells polarize in contact with these stromal cells by forming an anchoring structure that is reminiscent of immunological synapse. Our aim here is to study whether this polarization could be affected in leukemic cells, and perhaps impacting two key behaviors: anchorage versus migration and quiescence versus proliferation. The main hypothesis is that the \u201chematopoietic synapse\u201d prevents cell migration and maintains cell quiescence.<\/p><p>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 The experimental strategy is to analyze leukemic cells interactions with bone-marrow stromal cells using quiet innovative microfluidic devices that have been developed in our lab: a bone-marrow-on-a-chip that recapitulates both 3D structural and biochemical composition of endosteal and vascular niches. Indeed, the scheme consists on an investigation within the bone marrow emulation confronting leukemic stem cells (LSC) versus hematopoietic stem cells (HSC). Several models, comprising 1- Acute Myeloid Leukemia (AML) murine models, 2- AML human cell lines, 3- patient derived xenografts and 4- patient samples are to be used. It is planned to measure cell polarity and monitor cell behavior as well, using live-cell video-microscopy in the bone marrow-on-a-chip circuit. Thus, this potent tool will provide a quantitative measurement of LSC migration and proliferation in contact with stromal cells. Finally we will analyze whether LSC have an impact on the niche and whether the crosstalk between LSC and the niche could be druggable.<\/p><p>\u00a0<\/p><p>\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Agence Nationale pour la Recherche 18-IBHU-0003 (2019-2026) THEMA \u2013 Leukemia on Chip The global aim of this project is to decipher the cellular and molecular mechanisms regulating the interaction of leukemic blasts with bone-marrow stromal cells. We have recently found that hematopoietic stem cells polarize in contact with these stromal cells by forming an anchoring [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/pages\/3708"}],"collection":[{"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/comments?post=3708"}],"version-history":[{"count":5,"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/pages\/3708\/revisions"}],"predecessor-version":[{"id":3722,"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/pages\/3708\/revisions\/3722"}],"wp:attachment":[{"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/media?parent=3708"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}