{"id":3695,"date":"2022-09-08T16:13:21","date_gmt":"2022-09-08T14:13:21","guid":{"rendered":"http:\/\/cytomorpholab.com\/?page_id=3695"},"modified":"2022-09-08T16:28:59","modified_gmt":"2022-09-08T14:28:59","slug":"anr-sharp","status":"publish","type":"page","link":"http:\/\/cytomorpholab.com\/index.php\/anr-sharp\/","title":{"rendered":"ANR SHARP"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"3695\" class=\"elementor elementor-3695\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-77f0e13 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"77f0e13\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-468ea0f\" data-id=\"468ea0f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2cc5ed8 elementor-widget elementor-widget-text-editor\" data-id=\"2cc5ed8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.18.0 - 20-12-2023 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-aee81dd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"aee81dd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-fb3d591\" data-id=\"fb3d591\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-31540ec elementor-widget elementor-widget-image\" data-id=\"31540ec\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.18.0 - 20-12-2023 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t\t\t<img src=\"http:\/\/cytomorpholab.com\/wp-content\/uploads\/elementor\/thumbs\/logos-ANR_2022-pugo6ki60xr141aqjlwkegg9k9u99kb9ji8383hnyq.jpg\" title=\"logos-ANR_2022\" alt=\"logos-ANR_2022\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-66 elementor-top-column elementor-element elementor-element-1bab9a7\" data-id=\"1bab9a7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4f883c1 elementor-widget elementor-widget-text-editor\" data-id=\"4f883c1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h4>Agence Nationale pour la Recherche<\/h4>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-60a8d7d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"60a8d7d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6bb5e5a\" data-id=\"6bb5e5a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3d80a19 elementor-widget elementor-widget-text-editor\" data-id=\"3d80a19\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h4>AAPG2022 \u2013 PRC (2022-2026)<\/h4>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e719577 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e719577\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6613702\" data-id=\"6613702\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-47c1463 elementor-widget elementor-widget-text-editor\" data-id=\"47c1463\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h4>SHARP &#8211; Sharp Transition in the positioning of the microtubule-organizing center<\/h4>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-83747a6 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"83747a6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-8f1c304\" data-id=\"8f1c304\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-19bb113 elementor-widget elementor-widget-image\" data-id=\"19bb113\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" width=\"800\" height=\"250\" src=\"http:\/\/cytomorpholab.com\/wp-content\/uploads\/2022\/09\/ANR-Schema-1-1024x320.jpg\" class=\"attachment-large size-large wp-image-3406\" alt=\"\" srcset=\"http:\/\/cytomorpholab.com\/wp-content\/uploads\/2022\/09\/ANR-Schema-1-1024x320.jpg 1024w, http:\/\/cytomorpholab.com\/wp-content\/uploads\/2022\/09\/ANR-Schema-1-300x94.jpg 300w, http:\/\/cytomorpholab.com\/wp-content\/uploads\/2022\/09\/ANR-Schema-1-768x240.jpg 768w, http:\/\/cytomorpholab.com\/wp-content\/uploads\/2022\/09\/ANR-Schema-1.jpg 1260w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-ca80a83 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"ca80a83\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-7a2bc5c\" data-id=\"7a2bc5c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-63b8d4d elementor-widget elementor-widget-text-editor\" data-id=\"63b8d4d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Differentiated cells are polarized. A common and essential feature of polarized cells is the positioning of the centrosome, the main microtubule organizing center (MTOC), at the periphery of the cell, next to the active organelle (the flagellum of spermatozoa, the axon of neurons, the microvilli of epithelial cells, the immune synapse of lymphocytes). By contrast, in proliferating cells, it is located at the geometric center of the cell, hence its name, centrosome for &#8220;center of the soma&#8221;. The transition from one state to the other is not progressive. The regulation of the positioning of the MTOC goes from a robust and precise centering process to a total decentering to the periphery of the cell. The absence of intermediate states implies that the positioning mechanism is based on a nonlinear interaction between the forces acting on the microtubule network and the position of the center of this network, which induces a sharp transition in MTOC positioning. This sharp transition gives the cells both robust centering or decentering capabilities over wide ranges of conditions, as well as a highly sensitive state in a reduced window of parameters. The objective of this proposal is to study the physical basis of this complex regulation of the MTOC positioning mechanism by reconstructing in vitro all the key elements ensuring the different types of force production on microtubules, and to couple this experimental study to the establishment of a physical model capable of accounting for both the robust phases and the sharp transition.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1c84818 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1c84818\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-802ffa1\" data-id=\"802ffa1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Agence Nationale pour la Recherche AAPG2022 \u2013 PRC (2022-2026) SHARP &#8211; Sharp Transition in the positioning of the microtubule-organizing center Differentiated cells are polarized. A common and essential feature of polarized cells is the positioning of the centrosome, the main microtubule organizing center (MTOC), at the periphery of the cell, next to the active organelle [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/pages\/3695"}],"collection":[{"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/comments?post=3695"}],"version-history":[{"count":10,"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/pages\/3695\/revisions"}],"predecessor-version":[{"id":3719,"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/pages\/3695\/revisions\/3719"}],"wp:attachment":[{"href":"http:\/\/cytomorpholab.com\/index.php\/wp-json\/wp\/v2\/media?parent=3695"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}